The Establishment of Evaluation Index System for Emergency Response Capability among Health Supervision Institution / 中国卫生经济

Objective:To construct an evaluation indicator system for emergency response capability in health supervision institutions.Methods:It made an initial indicator system by using brain storming,literature method and expert consulting,and constructed an evaluation indicator system by Delphi method.Results:Both the response rates of two round questionnaires survey were 100％.The means of authoritative coefficient for the first-dimension indicators was 0.82.For the second round of expert consulting,the coordination coefficients of the first,second and third-dimension indicators were 0.954,0.820 and 0.565 respectively (P＜0.05).It constructed an evaluation indicator system for emergency response capability among health supervision institutions.There were 4 first-dimension indicators,10 second-dimension indicators and 37 third-dimension indicators in the indicators system.Conclusion:Experts opinions had high reliability.The process of screening indicators was normative.The indicator system was comprehensive,which could be used and examined in practice.

The Establishment of Evaluation Index System for Emergency Response Capability among Health Supervision Institution / 中国卫生经济

Objective:To construct an evaluation indicator system for emergency response capability in health supervision institutions.Methods:It made an initial indicator system by using brain storming,literature method and expert consulting,and constructed an evaluation indicator system by Delphi method.Results:Both the response rates of two round questionnaires survey were 100％.The means of authoritative coefficient for the first-dimension indicators was 0.82.For the second round of expert consulting,the coordination coefficients of the first,second and third-dimension indicators were 0.954,0.820 and 0.565 respectively (P＜0.05).It constructed an evaluation indicator system for emergency response capability among health supervision institutions.There were 4 first-dimension indicators,10 second-dimension indicators and 37 third-dimension indicators in the indicators system.Conclusion:Experts opinions had high reliability.The process of screening indicators was normative.The indicator system was comprehensive,which could be used and examined in practice.

Comparative Evaluations of in Vitro Biological Effects of PCL-R15/siRNA Nanoplexes Prepared by Different Processes / 中国药学杂志

OBJECTIVE: To prepare amphiphilic polycaprolactone-poly (arginine polymer) (PCL-R15)/siRNA Nanopexes, and two kinds of nanoparticles with different particle size were prepared by different process. After encapsulated siRNA with electrostatic interaction, both of two nanoplexes (NR60/siRNA and NR160/siRNA) were used to compare the effects in vitro cell levels. METHODS: The particle size and Zeta potential, siRNA loading and protection ability, cytotoxicity, cellular uptake mechanism and gene silencing efficiency of the two nanoplexes were investigated. RESULTS: The results show that the two nanoplexes have similar siRNA protection ability and cytotoxicity, but the difference between the two sizes is about 100 nm and the potential difference is about 20 mV. Moreover, NR160/siRNA complexes have higher cell uptake efficiency, more complex uptake pathways, and show greater gene silencing efficiency. CONCLUSION: These nanoplexes with different particle sizes can cause different transfection efficiency for siRNA delivery in cells.

Pharmacokinetics and relative bioavailability of ziprasidone tablets in Chinese healthy volunteers / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate the pharmacokinetics and bioavailability of ziprasidone tablets in Chinese healthy volunteers.</p><p><b>METHODS</b>A randomized crossover study was performed in 20 healthy volunteers, who received a single oral dose (40 mg) of the test or reference preparation of ziprasidone. Blood samples were collected from the subjects at different time points following the drug administration, and the plasma concentration of ziprasidone was determined using high-performance liquid chromatography. The pharmacokinetic parameters were analyzed by DAS software and the relative bioavailability was calculated according to the formula F=AUC(t)/AUC(r)x100%.</p><p><b>RESULTS</b>For the test and reference preparation, the pharmacokinetics parameter C(max) was 170.7-/+71.3 and 174.4-/+81.6 ng/ml, t(max) 3.73-/+1.87 and 3.69-/+1.84 h, t((1/2)) 5.57-/+1.62 and 5.61-/+1.73 h, AUC(0-t) 1273-/+252.3 and 1296-/+266.9 ng.h.ml(-1), and AUC(0-infinity)1396-/+276.9 and 1407-/+281.5 ng.h.ml(-1), respectively, with the relative bioavailability of (98.3-/+12.6)%. No significant differences were found in the main parameters of the test and reference preparations as analyzed by ANOVA and two- and one-side t-test.</p><p><b>CONCLUSION</b>The test and reference preparation of ziprasidone are bioequivalent.</p>

Pharmacokinetics of paroxetine tablet in healthy Chinese volunteers / 南方医科大学学报

<p><b>OBJECTIVE</b>To study the pharmacokinetics of paroxetine tablet in Chinese healthy volunteers.</p><p><b>METHODS</b>Twenty healthy subjects received a single oral dose of 40 mg paroxetine tablet. The plasma concentrations of paroxetine were determined using high-performance liquid chromatography (HPLC) and the measurements were analyzed with 3P97 program.</p><p><b>RESULTS</b>The plasma concentration curve of paroxetine following a single oral dose administration conformed to the two-compartment open model. The main pharmacokinetics parameters of paroxetine were: C(max)64.74-/+18.43 ng/ml, T(max)5.64-/+1.84 h, t(1/2) 20.03-/+5.33 h, AUC(0-120) 976.47-/+309.49 ng.h/ml, and AUC(0-inf) 1086.75-/+376.54 ng.h/ml.</p><p><b>CONCLUSION</b>The pharmacokinetics of paroxetine in human body conforms to the two-compartment open model.</p>

Reversed phase high-performance liquid chromatography for determination of plasma olanzapine and pharmacokinetics of olanzapine in healthy Chinese volunteers / 南方医科大学学报

<p><b>OBJECTIVE</b>To establish a reversed phase high-performance liquid chromatography (HPLC) system for determining plasma concentration of olanzapine and analyze the pharmacokinetics of olanzapine in healthy Chinese volunteers.</p><p><b>METHODS</b>Ten healthy male subjects received a single oral dose of 20 mg olanzapine tablets. The plasma concentrations of olanzapine were determined by HPLC, and the data were analyzed using 3P97 program.</p><p><b>RESULTS</b>The plasma concentration curve of olanzapine following a single oral dose conformed to the two compartment open model. The main pharmacokinetics parameters of olanzapine were as follow: C(max) was 113.7-/+33.1 microg/L, T(max) 5.07-/+0.65 h, t(1/2) 35.44-/+4.21 h, AUC(0-144) 2,235-/+257 microg.h.L(-1), and AUC(0-inf) 2,516-/+301 microg.h.L(-1).</p><p><b>CONCLUSION</b>The system established in this study allows for highly sensitive, selective and accurate determination of plasma concentration of olanzapine, and provides valuable information for clinical trials.</p>

Studies on the chemical constituents of a fungus producing perylenequinones / 药学学报

<p><b>AIM</b>To study the chemical constituents in the mycelia of Hypomyces sp..</p><p><b>METHODS</b>Silica gel column chromatography was employed for the isolation and purification. Chemical and spectral methods were used to determine the structures of the isolated compounds.</p><p><b>RESULTS</b>Two compounds were isolated and identified as: hypomycin C (I) and hypomycin D (II).</p><p><b>CONCLUSION</b>Compounds I and II are new compounds.</p>